ABSTRACT
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
Subject(s)
Animals , Dogs , Bone Matrix , Bone Substitutes/therapeutic use , Castor Oil/therapeutic use , Durapatite/therapeutic use , Mesenchymal Stem Cells/drug effects , Polyurethanes/therapeutic use , Tissue Scaffolds , Bone Regeneration/drug effects , Cell Adhesion , Cells, Cultured , Cell Differentiation/drug effects , Cell Proliferation/drug effects , Materials Testing , Microscopy, Electron, Scanning , Mesenchymal Stem Cells/cytology , Osteogenesis/drug effects , Surface Properties , Tissue EngineeringABSTRACT
Objective: To check the management of solid waste in dental practices that generates risks to health and the environment. With this in mind, the aim of this study was to ascertain the management of solid waste in private dental practices in the municipality of Quixadá, Ceará, Brazil in 2009. More specifically it was to ascertain its management, segregation, packing, collection, storage and final discording. Methods: This is a descriptive, exploratory and quantitative study. Out of a total of 15 dental practices in the municipality, 11 (73.3%) were included in the study. Data collection was conducted through a questionnaire applied to dentists in August 2009. Results: It was found that 81.8% of establishments do not have a Health Service Waste Management Plan. Nevertheless, 90.9% of professionals perform waste segregation, 45.5% of the dentists perform the packing of biological waste in plastic bags, 63.7% pack amalgam waste in glass with water, 60% dispose of developers and fixers directly into the sewerage system and for the sharps, 60% use cardboard boxes. Most dentists dispose of garbage on the sidewalk and the public collection is made by a truck, there being no separate collection service, and theywere transported to the landfill, where they do not receive the appropriate treatment. Conclusion: The lack of a Health Service Waste Management Plan leads to many failures and the involvement of the public authorities is essential in order to prevent harm to health and the environment.
Objetivo: Verificar o gerenciamento dos resíduos sólidos nos serviços odontológicos privados do Município de Quixadá, Ceará, no ano de 2009, mais especificamente, verificar esse gerenciamento, quanto à segregação, acondicionamento, coleta, armazenamento e destinação final.Métodos: Trata-se de um estudo descritivo, exploratório e predominantemente quantitativo. De um total de 15 serviços odontológicos do Município, 11 (73,3%) fizeram parte do estudo. A coleta de dados foi realizada por meio de um questionário, aplicado aos cirurgiões-dentistas, no mês de agosto de 2009. Resultados: Verificou-se que 81,8% dos estabelecimentos não possuem um Plano de Gerenciamento de Resíduos dos Serviços de Saúde, e ainda que 90,9% dos profissionais realizam a segregação dos resíduos e que 45,5% dos cirurgiões-dentistas realizam o acondicionamento dos resíduos biológicos em saco plástico comum, 63,7% acondicionam os resíduos de amálgama em vidros com água, 60% dispensam reveladores e fixadores diretamente na rede de esgoto e, para os resíduos perfurocortantes, 60% usam caixas de papelão. A maioria dos cirurgiões-dentistas acomoda o lixo na calçada e a coleta pública é feita por um caminhão, não havendo coleta diferenciada, sendo transportado para o aterro sanitário e sem tratamento correto. Conclusão: A falta do Plano de Gerenciamento de Resíduos dos Serviços de Saúde leva a muitas falhas e é importante a presença do poder público nosentido de prevenir os danos que podem causar à saúde e ao meio ambiente.
Subject(s)
Waste Management , Dental Waste , Solid WasteABSTRACT
PURPOSE: To evaluate three methods to isolate rats MSCs and to analyze the potential of a castor oil polyurethane base membrane as a scaffold for MSCs. METHODS: Four male Wistar rats, aged 20-30 days were used. Bone marrow aspirates from femur and tibia were harvested using DMEM high glucose and heparin. The cell culture was performed in three different ways: direct culture and two types of density gradients. After 15 days, was made the 1st passage and analyzed cell viability with markers Hoerscht 33342 and propidium iodide. The MSCs were characterized by surface markers with the aid of flow cytometry. After this, three types of castor oil polyurethane membranes associated with the MSCs were kept on the 6-well plate for 5 days and were analyzed by optical microscopy to confirm cell aggregation and growth. RESULTS: Separation procedures 1 and 2 allowed adequate isolation of MSCs and favored cell growth with the passage being carried out at 70 percent confluence after 15 days in culture. The cells could not be isolated using procedure 3. When the 3 castor oil polyurethane membrane types were compared it was possible to observe that the growth of MSCs was around 80 percent in membrane type 3, 20 percent in type 2, and 10 percent in type 1. CONCLUSION: Both Ficoll-Hypaque densities allow isolation of rat MSCs, and especially castor oil-based membrane type 3 may be used as a scaffold for MSCs.
OBJETIVO: Avaliar três formas de cultivo de células-tronco mesenquimais de ratos; e analisar o potencial do polímero de mamona na forma de membrana como arcabouço para CTMs. MÉTODOS: Foram utilizados quatro ratos machos Wistar, de 20 a 30 dias de idade. Aspirados da medula óssea do fêmur e da tíbia foram colhidos com DMEM alta glicose e heparina. As células foram isoladas de três formas diferentes: cultivo direto e com dois tipos de gradientes de densidade. Após 15 dias, foi feita a 1ª passagem e analisada a viabilidade celular com os marcadores Hoerscht 33342 e Iodeto de Propídio. As CTMs foram então caracterizadas por marcadores de superfície, com o auxílio de citômetro de fluxo. Após, três tipos de membrana à base de óleo de polímero de mamona associadas com as CTMS foram mantidas em cultivo por cinco dias, e analisados por microscópio ótico para confirmar o crescimento e a adesão celular. RESULTADOS: Após 15 dias, Os procedimentos que utilizaram gradientes de densidade permitiram o isolamento das CTMs e favoreceram o crescimento celular com a passagem, sendo obtido 70 por cento de confluência após 15 dias em cultura. O procedimento direto não se mostrou eficaz para o isolamento das células. O crescimento das CTMs foi aproximadamente 80 por cento sobre a membrana tipo 3, 20 por cento na tipo 2 e 10 por cento na membrana tipo 1. CONCLUSÃO: Os dois gradientes de concentração Ficoll-Hypaque permitem isolar CTMs de ratos; e especialmente a membrana de polímero de mamona tipo 3 pode ser usada como um bom arcabouço para as CTMs.